End-to-End Sequencing
We offer a comprehensive end-to-end RNA-seq service leveraging the Illumina NovaSeq® platform. We can take your sample from RNA extraction and purification through library prep and sequencing, and we facilitate access to cloud-based data analysis tool ROSALIND.
A Different Kind of Sequencing Provider
Because we are not a big-box sequencing provider, we take a collaborative and consultative approach to ensure your chosen method is appropriate for your sample type and study goals.
Easy Access to Complex Data
We partner with cloud-based bioinformatics platform ROSALIND to provide easy access to fast, dynamic, and visual data analysis tools to evaluate differential gene expression.
The Right Assay
Whether you are analyzing exclusively mRNA or cataloging all the transcripts in your sample, we can provide the suitable sequencing solutions for your goals and sample type.
Broad Services Portfolio
In addition to RNA-seq, we have an entire collection of technologies dedicated to single-cell and spatial transcriptomics analyses.
RNA-Seq Service Offerings
Your application and research goals will determine the optimal RNA-seq protocol. We offer three different workflows:
mRNA Sequencing
- Standard RNA-seq offering, targeting only coding species
- Poly-A enrichment library preparation
- Requires as little as 500 pg input
- Targeting 30 million paired end reads
Total RNA Sequencing
- Ideal for discovery work, targeting coding and non-coding RNA
- Ribosomal RNA (rRNA) depletion library preparation
- Requires as little as 25 ng input
- Targeting 30 million paired end reads
FFPE RNA Sequencing
- Total RNA-seq, with rRNA depletion library preparation
- Both Whole Transcriptome and Targeted Exome strategies available
- Deeper read depths to accommodate degraded samples, targeting 40 million paired end reads
How Our Service Works
RNA-Seq is a cost-effective, scalable method to investigate the transcriptome of a sample in response to treatment or to indicate disease. Our protocols follow a standard, transparent process to ensure you receive high-quality results every time.
Talk to Us
Our RNA-seq services are collaborative and customer-focused. Complete our RNA-seq request form to tell us about your project.
We Do the Work
RNA quantity and integrity is checked prior to library prep and sequencing Libraries are created and sequenced.
We Deliver Your Data
Receive raw FASTQ files or request cloud-based data analysis via ROSALIND.
Your Partner for Transcriptomics
Expand your transcriptomic experiments with our robust, quantitative targeted gene expression service using the nCounter® Analysis Platform. Gain more precise information from single cells using our scRNA-seq service.
RNA-Seq Gene Expression Analysis
Experience easy differential gene expression analysis with the ROSALIND cloud platform. Our RNA-seq service provides access to ROSALIND for powerful downstream analysis and insightful visualizations of gene expression data.
Simplify your analysis without the need for programming skills by using ROSALIND. Capable of advanced analyses, including covariate and batch correction, ROSALIND also generates interactive data visualizations and metabolic pathway interpretations.
Sample Requirements and Sequencing Depth
We have guidelines for sample inputs and standard parameters for our RNA sequencing. If your project falls outside of our recommendations, reach out and we will work with you to find a solution.
| Method | |||||||
|---|---|---|---|---|---|---|---|
| Library Type | |||||||
| Preferred Input (total RNA) | |||||||
| Acceptable Input (total RNA) | |||||||
| Stranded Library | |||||||
| Quality Recommendations | |||||||
| Organisms | |||||||
| Sequencing Depth (MM PE150 Read Pairs) | |||||||
| mRNA Seq | Poly-A selection | 100 – 1,000 ng | 25 ng – 100 ng | Yes | RIN > 7.0 | All Eukaryotes | 30 |
| Total RNA Seq | rRNA/globin depletion | 100 ng – 1,000 ng | 25 ng – 100 ng | Yes | RIN > 2.0 | Human, Mouse, Rat, and Bacteria | 30 |
| Low Input mRNA Seq | Poly-A selection | 0.1 ng – 10 ng | 0.05 ng – 0.1 ng | No | RIN > 7.0 | All Eukaryotes | 30 |
| FFPE Total RNA Seq | rRNA depletion | 200 ng – 1,000 ng | 100 ng – 200 ng | Yes | %DV200 > 20% | Human, Mouse, Rat | 40 |
Orthogonal Validation: Conclude with Confidence
Generate discoveries and create hypotheses leveraging our RNA-seq services, and then expand upon and verify your insights with our CellScape™ Service. Our exclusive Precise Spatial Proteomics imaging technology can both detect and quantify a virtually unlimited number of biomarkers from your tissue or suspension cell samples while maintaining high resolution spatial information. Partner with us and take your concept to conclusion.
Frequently Asked Questions
Yes, we also offer single-cell RNA-Seq services using the Chromium® X from 10X Genomics.
We can accept total RNA, or we can do the RNA extraction from your preferred sample type, including cell pellets, fresh frozen tissue, FFPE blocks and slides, and blood.