VistaPlex™ Tissue Architecture Assay Kit
Modular Multiplexing for the CellScape™ Platform
Achieve characterization of key structural features in human FFPE samples with spatial context using the Tissue Architecture Assay Kit.
AbKT-3002-10RXN
Spatial Discovery Builds Here
Providing a modular building block for essential spatial discovery of key structural features of human FFPE samples, the Tissue Architecture Assay Kit includes a panel of antibodies curated and optimized by our robust validation workflow. The panel targets key epithelial, lymphatic, lamina, vasculature and connective tissue biomarkers and can be expanded by pairing with additional VistaPlex assay kits.
Spatially profile the immune landscape with 10 biomarkers and nuclear stain
| CD138 | SMA | CD31 | Collagen IV |
| Podoplanin | Beta-catenin | CD34 | E-Cadherin |
| Vimentin | MUC1 | DAPI | |
The combination of these markers allows researchers to visualize key structural features of human FFPE samples.
Convenient and Efficient Spatial Multiplexing
VistaPlex Tissue Architecture Assay Kit includes the necessary reagents and protocol to stain up to 10 human FFPE samples with 10 biomarkers using CellScape automated staining and imaging.
Validated Antibodies
A panel of validated fluorescently labeled antibodies for 10 biomarkers is included for identifying structural tissue features.
Reagents and Buffers
Antibody diluent is included for preparation of antibody cocktails. DNA stains are included for cell segmentation.
Optimized Protocol
An optimized protocol is included to guide execution and analysis of the multiplexed assay.
Validation Ensures Reliable Antibody Panel Performance
Antibodies for the multiplex panel were validated using three human FFPE tissue samples in triplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability.
Data Summary
| Tissue Type | |||
|---|---|---|---|
| Suitability | |||
| Specificity | |||
| Reproducibility | |||
| Normal Gallbladder FFPE |
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| Normal Tonsil FFPE |
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| Normal Prostate FFPE |
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| Normal Appendix FFPE |
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| Normal Testis FFPE |
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| Normal Lymph Node FFPE |
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| Normal Ovary FFPE |
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| Breast Cancer FFPE |
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Not Tested |
Ready to Accelerate your CellScape Research?
Expedite your immunophenotyping with the efficient and reliable VistaPlex Tissue Architecture Assay Kit: AbKT-3002-10RXN.
Need Higher Plex?
Expand the Spatial Immune Profiling Assay Kit with additional modular VistaPlex Assay Kits. Our multiplex kits for the same sample types are all suitable for use with each other. Add additional biomarkers from our large library of CellScape Compatible Biomarkers, or use your own unique antibodies to achieve a Precise Spatial Multiplexing panel that fits your needs.
Explore our Assay Solutions
Browse More VistaPlex Assay Kits
Learn more about how our catalog of VistaPlex Assay kits can expedite development and accelerate precise data generation for CellScape multiplexed assays.
Leverage our CellScape Services
We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.
Additional CellScape Products Needed
Microfluidic chambers for FFPE tissues, product PRSM-CHP-TISSUE, are required for loading samples for staining with the VistaPlex Assay Kits. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles.
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