Bruker Announces EpicIF™, Revolutionary Fluorescence Signal Removal Technology, Transforming CellScape Spatial Proteomics Platform
New Technology Expands Antibody Compatibility and Doubles Throughput while maintaining Tissue Integrity and Lack of Cross-Reactivity
BILLERICA, Massachusetts—October 28, 2024—Bruker Corporation today announced a major technological breakthrough in the CellScape™ Precise Spatial Proteomics platform for highly multiplexed immunofluorescence (IF). Launched in 2022, the CellScape platform has advanced the field of spatial proteomics with differentiated quantitative performance enabled by a unique combination of best-in-class resolution and high dynamic range (HDR) imaging. CellScape captures the entire continuum of protein expression in a biological sample—from least to most abundant—while capturing fine morphological details. CellScape utilizes directly-labeled primary antibodies, enabling a robust and modular chemistry that allows researchers to build assays by combining panels and/or individual markers, even after the conclusion of an experimental run. With this new technology, Bruker has further advanced the CellScape chemistry with EpicIF (Enhanced photobleaching in cyclic immunofluorescence), which expands the range of compatible commercially available fluorophore conjugated antibodies by nearly 10-fold, simplifies assay development, and increases throughput by up to 2-fold. EpicIF enhances photobleaching efficacy combining a proprietary reagent with visible light to gently erase fluorescence signal from nearly any fluorophore and, like the prior version, the epitopes are preserved while maintaining tissue integrity.
EpicIF is supported by the concurrent release of a new version of the software. The new software, CellScape™ Navigator, also introduces a more intuitive user interface and easy experiment setup. Both EpicIF and CellScape Navigator will be available as upgrades to current CellScape instruments.