Analyze Gene Expression at the Single Cell Level
Resolve the complex cellular diversity of your samples with our single-cell RNA Sequencing (scRNA-Seq) service. Exploring cellular heterogeneity reveals gene expression patterns and identifies distinct cell types and interactions.
For use in biomarker discovery, the gene expression profiles of individual cells can highlight genes or markers associated with cell types in specific disease states. Partner with our scientific team to understand the complexity within your samples and gain impactful results.
Expertise and Experience
Our breadth of technology platforms demonstrates our scientific capabilities – in handling your precious samples, offering experimental design feedback, executing your project, and providing quality data.
Easy Access to Complex Data
Gain powerful insights into cellular heterogeneity and function by pairing single-cell sequencing with simple and accessible bioinformatic analysis from our partner, ROSALIND.
Unraveling Cellular Diversity
10x Chromium X scRNA-seq offers powerful insights into the complexity of cellular diversity. Our workflow results in fast project turnaround so you can unravel these insights.
Comprehensive Portfolio
With access to the newest technologies and the expertise to support your unique project needs, we provide a convenient end-to-end solution for single-cell RNA sequencing.
Bringing Single-Cell Solutions Together
Complement your scRNA-Seq transcriptomic data with our CellScape™ Precise Spatial Proteomics service or our standard immunofluorescence service to verify changes in target protein expression.
How Our Service Works
Our scRNA-seq workflow uses the 10x Chromium X instrument to isolate single cells from cell pellets or tissue samples. Our internal processes efficiently assess input sample quality and any dead cell removal required. From there, it's three simple steps from processing to delivery of your data.
Sample Processing
The Chromium X scRNA-seq platform isolates cells and provides single-cell gene expression information to profile up to tens of thousands of cells.
Sequencing
High-throughput sequencing is done using Illumina NextSeq® technology. The output can be adjusted based on your project need.
Data Analysis
Comprehensive and interactive data output using the ROSALIND web-based platform.
How We Approach The scRNA-Seq Workflow
Single Cell Sample Input
Sample viability is a critical component of the input for any scRNA Seq assay. Key sample types include cryopreserved PBMCs, single cell suspensions, and fresh frozen and FFPE tissue. We assess cell viability post thaw and samples with less than 80% viability will undergo dead cell removal. If submitting tissue, we will do a QC check to ensure successful nuclei retrieval.
Profiling Assay for Gene Expression
We offer several 10x workflows; select the appropriate one for your study goals.
Data Interpretation
Access to ROSALIND is provided for data transfer and visualization. This easy-to-use, cloud-based platform allows for interactive analysis of single cell clusters. We can support your analysis with a platform tutorial.
New Insights With Single-cell Transcriptomics
Single-cell RNA-seq allows for gene expression profiling of thousands of individual cells. The advantage of this technique is that researchers can more precisely understand and explore cellular diversity in complex systems by interrogating gene expression of individual cells. This high-resolution technique can uncover subtle differences between cells, often masked in bulk RNA sequencing, offering a better understanding of cellular diversity, disease states and developmental biology.
Visualize and Interpret Gene Expression
Analyze your single-cell sequencing results with a fully automated process using the ROSALIND cloud-based tool. Intuitive workflows and visualization tools are available for single-cell transcriptome data analysis. Highlights of using ROSALIND include:
- Cluster Comparisons
- Data Visualization
- Pathway Interpretation
Frequently Asked Questions
Single-cell RNA sequencing, or scRNA-seq, is a specialized technique that allows researchers to analyze the complete, unbiased transcriptional profiles of individual cells from a cell suspension or tissue sample. The technology makes it possible to identify and characterize the diversity of normal and degraded cells. Single-cell RNA sequencing gives transcriptomic information that can be analyzed using bioinformatics tools to identify cell-specific gene expression patterns.
We offer the 3’ Single Cell Transcriptomics (v3.1) and Single Cell Immune Profiling with TCR and BCR V(D)J analysis using 10X Genomics kits and reagents.
Canopy Multiomic Services requires a minimum of four samples per project, with a concentration of at least 2 million cells/mL per sample.
Please send cryopreserved cells using the appropriate cryopreservation medium. We recommend supplementing your cell culture media with 20% Fetal Bovine Serum (FBS) and 10% Dimethyl Sulfoxide (DMSO).
High sample viability is critical to every scRNA-seq experiment. We request that your cells have a sample viability of 80% post-thaw. If your samples have less than 80% viability, we will remove the dead cells for you.
The number of cells you can expect per sample depends on your experimental goals. Our standard service returns data for approximately 5,000 cells per sample. Contact our scientists to discuss any further inquiries.
Our standard service includes 50,000 reads per cell.