# 2 Study Design

We can summarize the study design in a Sankey diagram. This visualization is useful for showing the flow of samples and how they relate to biological annotations. Individual ROI/AOI segments are annotated by slide name, class, region, and segment. Class refers to disease status.

The width of a cord in the figure represents how many segments are in common between the two annotations they connect. 14 segments were removed from the study due to QC flags or low target detection rates, as shown in the QC column below. For more detail on segment QC please see the next section.