Samples were collected at 3 different timepoints throughout trial (pretreatment, after 1 round therapy, after 5 rounds therapy). There was an increase in HR associated signatures at Time 3 vs the first (ER signaling at Time 2 and PGR at Time 2 and Time 3). Furthermore, PD-L2 was upregulated at both Times 2 and 3, and was higher in the responders. The claudin low signatures increased at both Times 2 and 3, but the increase was much more dramatic in non-responders.