VistaPlex™ T-Cell Profiling Assay Kit
Multiplexing for the CellScape™ Platform
Accelerate your CellScape quantitative data generation, and achieve phenotyping of T cells in human cell suspension samples, including PBMCs, using the T Cell Profiling Assay Kit.
AbKT-1003-10RXN
Immune Discovery Starts Here
The T Cell Profiling Assay Kit enables quantification and characterization of 8 key T cell biomarkers for in-depth single-cell phenotyping in human PBMC samples. The optimized panel of antibodies has been tested following our robust validation workflow to ensure reproducible performance.
Profile the immune landscape with 8 biomarkers
| CD3 | CD25 | CD39 | CD8 |
| CD4 | CD27 | CD45RA | CD45 |
The combination of these markers allows researchers to phenotype key immune cell populations.
Single Cell Phenotyping and Quantification for at least 13 Immuno-Phenotypes
| Leukocytes | T cells | Cytotoxic T cells | Helper T cells |
| Regulatory T cells | Naive CD4+ cells | Central memory CD4+ cells | Effector CD4+ cells |
| Effector memory CD4+ cells | Naive CD8+ cells | Central memory CD8+ cells | Effector CD8+ cells |
| Effector memory CD8+ cells | |||
Convenient and Efficient Immunophenotyping
VistaPlex T Cell Profiling Assay Kit includes the necessary reagents and protocol to stain up to 10 human PBMC samples with 8 biomarkers using CellScape automated staining and imaging.
Validated Antibodies
A panel of validated fluorescently labeled antibodies for 8 biomarkers is included for immunophenotyping.
Reagents and Buffers
Antibody diluent is included for preparation of antibody cocktails.
Optimized Protocol
An optimized protocol and a phenotyping strategy are included to guide execution and analysis of the multiplexed assay.
Validation Ensures Reliable Antibody Panel Performance
Antibodies for the multiplex panel were validated using 3 different blood donors in duplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability.
To ensure high quality data generation from a variety of sample types, the assay kit was successfully tested on samples that were biobanked on CellScape Slides for 6 months as well as cryopreserved samples. Download the validation report for experiment details and results.
Data Summary Table
| Sample Type | |||
|---|---|---|---|
| Suitability | |||
| Specificity | |||
| Reproducibility | |||
| Fresh CPT PBMCs |
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| Cyto-Chex® BCT PBMCs |
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| Cryopreserved PBMCs |
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Ready to Accelerate your CellScape Research?
Expedite your next immunophenotyping project with the efficient and reliable VistaPlex T Cell Profiling Assay Kit: AbKT-1003-10RXN.
Need Higher Plex?
Create an antibody panel that suits your experimental needs and maximizes the impact of your research. Expand the T Cell Profiling Assay Kit by adding additional biomarkers from our large library of CellScape Compatible Biomarkers, or use your own unique antibodies to achieve a multiplexing panel that fits your needs.
Explore our Assay Solutions
Browse More VistaPlex Assay Kits
Learn more about how our catalog of VistaPlex Assay kits can expedite development and accelerate precise data generation for CellScape multiplexed assays.
Leverage our CellScape Services
We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.
Additional CellScape Products Needed
Microfluidic chambers for cell suspensions, product PRSM-CHP-CELL, are required for loading samples for staining with the T Cell Profiling Assay Kit. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles.
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