VistaPlex™ Fresh Frozen Immune Profiling Assay Kit

Foundational Multiplexing for the CellScape™ Platform

Achieve fundamental spatial immunophenotyping of the tumor microenvironment in human fresh frozen tissue samples using the Fresh Frozen Immune Profiling Assay Kit.

AbKT-2001-10RXN

VistaPlex Spatial Immune Profiling Kit

Spatial Discovery Starts Here

Spatially profile the immune landscape with 13 biomarkers and nuclear stain

HLA-DR CD56 CD45RA CD8
CD123 CD14 CD20 Nuclear Stain
CD4 CD27 CD45
PanCytokeratin CD11c CD3
Hu-FF-appendix-with-legend@2x

The combination of these markers allows researchers to phenotype key tumor and immune cell populations.

Single-Cell Phenotyping, Spatial Context, and Quantification for at least 23 Immuno-Phenotypes

Nucleated cells Leukocytes Epithelia NKT cells
NK cells Memory B cells T cells Helper T cells
Cytotoxic T cells Central Memory CD4+ cells Naive CD4+ cells Effector CD4+ cells
Effector Memory CD4+ cells Central Memory CD8+ cells Naive CD8+ cells Effector CD8+ cells
Effector Memory CD8+ cells B cells Naive B cells Monocytes
HLA DR+ dendritic cells Plasmacytoid dendritic cells Myeloid dendritic cells

Convenient and Efficient Spatial Multiplexing

Validated Flourescently labled antibodies-logo

Validated Antibodies

A panel of validated fluorescently labeled antibodies for 13 biomarkers is included for immunophenotyping.

Reagents and Buffers-logo

Reagents and Buffers

Antibody diluent is included for preparation of antibody cocktails. A DNA stain is included for cell segmentation.

Validation Ensures Reliable Antibody Panel Performance

Antibodies for the multiplex panel were validated using three human fresh frozen tissue samples in duplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability. Download the validation report for experiment details and results.

Data Summary

Tissue Type
Suitability
Specificity
Reproducibility
Breast FF
Colon FF
Lung FF
Appendix FF

Ready to Accelerate your CellScape Research?

Expedite your immunophenotyping with the efficient and reliable VistaPlex Fresh Frozen Immune Profiling Assay Kit: AbKT-2001-10RXN.

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Leverage our CellScape Services

We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.

Additional CellScape Products Needed

Microfluidic chambers for tissues, product PRSM-CHP-TISSUE, are required for loading fresh frozen samples for staining with the VistaPlex Assay Kits. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles.

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