VistaPlex™ Lymphoid and Myeloid Profiling Assay Kit

Multiplexing for the CellScape™ Platform

Accelerate your CellScape quantitative data generation and achieve lymphoid and myeloid cell phenotyping in human cell suspension samples, including PBMCs, using the Lymphoid and Myeloid Immune Profiling Assay Kit.

AbKT-1001-10RXN

VistaPlex Human PBMC Assay Kits

Immune Discovery Starts Here

Profile the immune landscape with 12 biomarkers

CD3 CD25 CD39 CD8
CD4 CD27 CD45RA CD45
CD56 CD16 CD14 CD19
12plex-lymphoid-myeloid-PBMC-with-legend

The combination of these markers allows researchers to phenotype key immune cell populations.

Single Cell Phenotyping and Quantification for at least 20 Immuno-Phenotypes

Leukocytes T cells Cytotoxic T cells Helper T cells
Regulatory T cells Naive CD4+ cells Central memory CD4+ cells Effector CD4+ cells
Effector memory CD4+ cells Naive CD8+ cells Central memory CD8+ cells Effector CD8+ cells
Effector memory CD8+ cells NK cells NKT cells B cells
Memory B cells Naive B cells Classical monocytes Non-classical monocytes

Convenient and Efficient Immunophenotyping

Antibody no circle

Validated Antibodies

A panel of validated fluorescently labeled antibodies for 12 biomarkers is included for immunophenotyping.

Reagents and Buffers

Reagents and Buffers

Antibody diluent is included for preparation of antibody cocktails.

Validation Ensures Reliable Antibody Panel Performance

Antibodies for the multiplex panel were validated using 3 different blood donors in duplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability.

To ensure high quality data generation from a variety of sample types, the assay kit was successfully tested on samples that were biobanked on CellScape Slides for 6 months as well as cryopreserved samples. Download the validation report for experiment details and results.

Data Summary Table

Sample Type
Suitability
Specificity
Reproducibility
Fresh CPT PBMCs
Cyto-Chex® BCT PBMCs
Cryopreserved PBMCs

Ready to Accelerate your CellScape Research?

Expedite your next immunophenotyping project with the efficient and reliable VistaPlex Lymphoid and Myeloid Profiling Assay Kit: AbKT-1001-10RXN.

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Leverage our CellScape Services

We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.

Additional CellScape Products Needed

Microfluidic chambers for cell suspensions, product PRSM-CHP-CELL, are required for loading samples for staining with the Lymphoid and Myeloid Profiling Assay Kit. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles.

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