VistaPlex™ Spatial Immune Profiling Assay Kit
Foundational Multiplexing for the CellScape™ Platform
Achieve fundamental spatial immunophenotyping of the tumor microenvironment in human FFPE samples using the Spatial Immune Profiling Assay Kit.
VISTAPLEX-3102-10RXN
Spatial Discovery Starts Here
Providing a building block for essential spatial discovery of the immune microenvironment, the Spatial Immune Profiling Assay Kit includes a panel of antibodies curated and optimized by our robust validation workflow. The panel targets key immune biomarkers and can be expanded by pairing with additional VistaPlex assay kits.
Spatially profile the immune landscape
| Marker | |||
|---|---|---|---|
| Cell Type | |||
| Marker | |||
| Cell Type | |||
| CD20 | B cells | CD8 | Cytotoxic T cells |
| CD3 | T cells and NKT cells | CD45RO | Activated and memory T cells |
| CD279 | Exhausted T cells | CD38 | plasma cells |
| CD274 | Immunosuppressive cells | Ki-67 | Proliferating cells |
| FoxP3 | T Regs | CD68 | Macrophages, myeloid cells |
| CD4 | Helper T and T Regs | CD45RA | Naive and effector t cells |
| CD45 | Immune cells | PanCK | epithelial cells |
| CD19 | B cells | CD163 | Macrophages, myeloid cells |
| Granzyme B | Activated cytotoxic T cells and NK cells |
Single Cell Phenotyping, Spatial Context, and Quantification for at least 24 Immuno-Phenotypes
| Nucleated Cells | Leukocytes | Epithelia | Macrophages |
| T Cells | Helper T Cells | Cytotoxic T Cells | T Regulatory Cells |
| Proliferating CD4+ Cells | Exhausted CD4+ Cells | Central Memory CD4+ Cells | Naive CD4+ Cells |
| Effector CD4+ Cells | Effector Memory CD4+ Cells | Granzyme B+ CD8+ T Cells | Proliferating CD8+ Cells |
| Exhausted CD8+ Cells | Central memory CD8+ Cells | Naive CD8+ Cells | Effector CD8+ Cells |
| Effector Memory CD8+ Cells | B Cells | Memory B Cells | Naive B Cells |
Convenient and Efficient Spatial Multiplexing
The VistaPlex Spatial Immune Profiling Assay Kit enables automated staining and imaging of 10 human FFPE samples for 17 biomarkers using the CellScape Precise Spatial Proteomics platform.
Validated Antibodies
The kit includes 17 validated, fluorescently labeled antibodies to support tissue immunophenotyping.
Reagents and Buffers
Antibody diluent is provided for preparing antibody cocktails; EpicIF Buffer and Wash Buffer are available separately.
Optimized Protocol
An online protocol is available to guide consistent and reliable execution of the multiplexed assay.
Validation Ensures Reliable Antibody Panel Performance
Antibodies for the multiplex panel were validated using eight human tonsil FFPE tissue samples to ensure specificity, sensitivity, and reproducibility. Additional FFPE tissue types were validated for broad sample suitability. Download the validation report for experiment details and results.
Data Summary
| FFPE Tissue Type | |
|---|---|
| Suitability | |
| Tonsil |
|
| Breast Cancer |
|
| Colon Cancer |
|
| Melanoma |
|
| Head and Neck Cancer |
|
Ready to Accelerate your CellScape Research?
Expedite your immunophenotyping with the efficient and reliable VistaPlex Spatial Immune Profiling Assay Kit: VISTAPLEX-3102-10RXN.
Need Higher Plex?
Expand the Spatial Immune Profiling Assay Kit with modular VistaPlex Assay Kits to build a customized Precise Spatial Proteomics panel. Compatible kits can be run on the same sample, and the CellScape workflow lets you seal, store, and revisit the same slide. Add new biomarkers later to drive data-driven panel expansion and make the most of precious samples.
Explore our Assay Solutions
Browse More VistaPlex Assay Kits
Learn more about how our catalog of VistaPlex Assay kits can expedite development and accelerate precise data generation for CellScape multiplexed assays.
Leverage our CellScape Services
We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.
Additional CellScape Products Needed
Whole Slide Imaging Chambers, product PRSM-CS-WSIC, are required for converting slides into microfluidic chambers required for staining with the VistaPlex Assay Kits. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles. EpicIF Buffer, product PRSM-BUF-EPIC, is required for signal removal between cycles.
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