VistaPlex™ Spatial Immune Profiling Assay Kit

Foundational Multiplexing for the CellScape™ Platform

Achieve fundamental spatial immunophenotyping of the tumor microenvironment in human FFPE samples using the Spatial Immune Profiling Assay Kit.

AbKT-3001-10RXN

VistaPlex Spatial Immune Profiling Kit

Spatial Discovery Starts Here

Spatially profile the immune landscape with 14 biomarkers and nuclear stain

CD3 Granzyme B Ki-67 panCK
CD45RA FoxP3 CD68 CD45RO
PD-1 CD8 PD-L1 Nuclear Stain
CD4 CD20 CD45
FFPE-Immune-Profiling-with-legend

The combination of these markers allows researchers to phenotype key tumor and immune cell populations.

Single Cell Phenotyping, Spatial Context, and Quantification for at least 24 Immuno-Phenotypes

Nucleated Cells Leukocytes Epithelia Macrophages
T Cells Helper T Cells Cytotoxic T Cells T Regulatory Cells
Proliferating CD4+ Cells Exhausted CD4+ Cells Central Memory CD4+ Cells Naive CD4+ Cells
Effector CD4+ Cells Effector Memory CD4+ Cells Granzyme B+ CD8+ T Cells Proliferating CD8+ Cells
Exhausted CD8+ Cells Central memory CD8+ Cells Naive CD8+ Cells Effector CD8+ Cells
Effector Memory CD8+ Cells B Cells Memory B Cells Naive B Cells

Convenient and Efficient Spatial Multiplexing

Antibody no circle

Validated Antibodies

A panel of validated fluorescently labeled antibodies for 14 biomarkers is included for immunophenotyping.

Reagents and Buffers

Reagents and Buffers

Antibody diluent is included for preparation of antibody cocktails. DNA stains are included for cell segmentation.

Validation Ensures Reliable Antibody Panel Performance

Antibodies for the multiplex panel were validated using three human FFPE tissue samples in duplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability. Download the validation report for experiment details and results.

Data Summary

Tissue Type
Suitability
Specificity
Reproducibility
Breast FFPE
Colon FFPE
Lung FFPE
Ovary FFPE Not tested
Spleen FFPE Not tested
Tonsil FFPE Not tested

Ready to Accelerate your CellScape Research?

Expedite your immunophenotyping with the efficient and reliable VistaPlex Spatial Immune Profiling Assay Kit: AbKT-3001-10RXN.

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Leverage our CellScape Services

We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.

Additional CellScape Products Needed

Microfluidic chambers for FFPE tissues, product PRSM-CHP-TISSUE, are required for loading samples for staining with the VistaPlex Assay Kits. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles.

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